Title: Cleans and Normalizes DBF files obtained from experiments using the FLUOstar microplate reader.
Source:R/normfluordbf.R
normfluordbf.Rd
Input the path to a ".dbf" file obtained from the FLUOstar microplate (usually a 96-well microplate) reader; this function will create a data frame, clean the data frame, normalize the data frame, append a "Cycle_Number" column and return a data frame that is ready for analysis. Most importantly, this function is a single_step function. Also, the function can be extended to other ".dbf" files if they follow the format for which this function was designed; this is totally at the users' discretion.
Arguments
- file
A string ("liposomes_xxx.dbf") if the file is found within the present working directory (pwd) OR a path pointing directly to a ".dbf" file, from FLUOstar experiments.
- norm_scale
This parameter can taken in 'hundred', 'one', or 'z-score' which denotes the normalization type; Initialized as NULL.
- transformed
This parameter can take in 'log' which denotes a logarithmic box-cox transformation; Initialized as NULL.
- fun
A variable defined as NA, used for boolean expressions or manipulation.
- ...
A container object that can be used to capture extra variables if needed.
Note
Re-nomenclature of norm_tidy_dbf to a more appropriate name that facilitates function utilization. Users can continue with the old name ("norm_tidy_dbf") but this is a better name in my opinion.
Examples
fpath <- system.file("extdata", "liposomes_214.dbf", package = "normfluodbf", mustWork = TRUE)
normalized_dbf <- normfluordbf(file=fpath, norm_scale = 'raw')
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#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 4
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#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 4
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#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 4
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#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 5
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#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 5
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#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 6
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#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 7
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#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 7
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#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 7
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#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 8
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#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 18
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#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 18
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#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 18
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#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 20
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#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 20
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#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 20
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#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 20
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#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 21
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#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 21
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#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 21
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#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 21
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#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 21
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#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 21
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#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 21
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#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 21
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#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 22
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#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 22
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#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 22
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#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 22
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#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 22
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#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 22
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#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 22
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#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 22
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#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 22
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#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 23
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#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 23
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#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 23
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#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 23
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#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 23
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 23
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 23
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 23
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 23
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 24
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 24
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 24
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 24
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 24
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 24
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 24
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 24
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 24
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 25
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 25
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 25
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 25
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 25
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 25
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 25
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 25
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 25
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 26
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 26
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 26
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 26
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 26
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 26
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 26
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 26
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 26
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 27
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 27
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 27
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 27
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 27
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 27
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 27
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 27
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 27
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 28
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 28
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 28
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 28
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 28
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 28
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 28
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 28
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 28
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 29
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 29
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 29
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 29
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 29
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 29
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 29
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 29
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 29
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 30
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 30
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 30
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 30
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 30
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 30
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 30
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 30
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 30
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 31
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 31
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 31
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 31
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 31
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 31
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 31
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 31
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 31
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 32
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 32
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 32
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 32
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 32
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 32
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 32
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 32
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 32
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 33
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 33
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 33
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 33
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 33
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 33
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 33
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 33
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 33
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 34
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 34
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 34
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 34
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 34
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 34
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 34
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 34
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 34
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 35
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 35
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 35
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 35
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 35
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 35
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 35
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 35
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 35
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 36
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 36
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 36
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 36
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 36
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 36
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 36
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 36
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 36
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 37
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 37
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 37
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 37
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 37
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 37
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 37
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 37
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 37
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 38
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 38
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 38
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 38
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 38
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 38
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 38
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 38
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 38
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 39
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 39
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 39
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 39
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 39
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 39
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 39
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 39
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 39
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 1 row: 40
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 2 row: 40
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 3 row: 40
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 4 row: 40
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 5 row: 40
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 6 row: 40
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 7 row: 40
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 8 row: 40
#> 🐷🐫🦁
#> YIKES, value > 2^15, Watch in future experimental designs column: 9 row: 40